The isolation and regeneration of potato (Solanum tuberosum L) protoplasts have been carried out. Mesophyl cell protoplast were isolated from two dihaploid . The Isolation of regeneratiom of potato (Solanum tuberosum L.) protoplasts have been carried out. Mesophyl cell protoplast were isolated from two dihaploid. One of new technologies in order to create hybrid plant from different species is protoplast fusion. This technique involves protoplast isolation, purification.

Author: Zululkree Kejas
Country: Guatemala
Language: English (Spanish)
Genre: Sex
Published (Last): 2 December 2015
Pages: 324
PDF File Size: 17.68 Mb
ePub File Size: 5.1 Mb
ISBN: 986-4-41350-313-7
Downloads: 24781
Price: Free* [*Free Regsitration Required]
Uploader: Kigatilar

Agriculture General Country of publisher: Fusion was induced with PEG solution according to Uchimia and fused protoplasts were collected by centrifugation of g.

Isolation of protoplast from soybean, cowpea, and tobacco and their fusion. Standard Search Advanced Search. Data were analyzed using Kruskal-Wallis test followed by Mann-Whitney test. The obyectives of the research was to investigate the effect of combination treatment of sucrose concentrations and incubation time prooplas protoplast isolation of the leaves mesophyll orchid of Paraphalaenopsis layockii.

The highest protoplast density and number of viable protoplasts achieved in the combined treatment of 0.

On both medium leaf protoplast, irradiated protoplasts and their fused do not regenerate cell rpotoplas and all cultured died out within four weeks incubation. The first factor was concentrations of sucrose which consists of three levels namely 0.


Isolation of protoplast from soybean, cowpea, and tobacco and..|INIS

Indonesia Language of fulltext: Leaf protoplast were releasaed in h, soybean and tobacco callus protoplast in h, and cowpeas callus protoplast in h of incubation. Javascript must be enabled for narrowing.

Journal on Agriculture Science. Funding for sustainable Open Access incl. Protoplast were cultured on the medium of Ikeda and Uchimia. The results showed that there was effect of treatment for all variables observed. Results 1 – 1 of 1. Looks like Javascript is disabled on your browser. Callus wich has been two times resubcultured was suspended in the digestive medium without driselase, CaCl2, and MES and incubated in lowlight intensity by constant shaking at rpm in reciprocal water shaker at 30 celcius centigrade.

Journal Homepage Journal Title: The factorial experiment consisted of two factors that was arranged by completely randomized design CRD with ten replications. Indonesian, English Full-text formats available: Our members Our publisher members Our sponsors Our volunteers.

Prosiding Seminar Nasional Biologi

Reference Number Reference Number. They were incibated in full darkness at 27 Celcius centigrade by constant shaking at 50 rpm orbitor shaker.


Regeneration of cell wall observed progessively in mother protoplast from tobacco, cowpea A27, E1, and H6 and fused protoplast of soybean with tobacco, tobacco with cowpea C4, E1, and H6soybean with cowpea C4 and between cowpea C4 and cowpea E1. Close Go to Workspace. Please provide a name for this query: Cell wall generation was observed. Get permanent URL for this record.

Isolasi Protoplas secara Enzimatis pada Tanaman Kecipir | Riyadi | Buletin Plasma Nutfah

Protoplast were collected by centrifugation of g and a thin layer of the suspension was irradiated with ultraviolet light. Isolasi dan fusi protoplas dari kedelai, buncis, dan tembakau. For soybean leaf, the medium was enriched with driselase and pectolyase Y The second factor was incubation time which consists of four levels namely 2 hours, 4 hours, 6 hours and 8 hours.

Publication Year Publication Year.